Capto resin6/12/2023 ![]() Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values. Wash with at least 2 CV ultra pure water.ĥ. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.Ĥ. Stable to commonly used aqueous buffers, 1.0 M NaOH 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.ġ. This prevents clogging and increases the life time of the column when loading large sample volumes. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. Regular CIP prevents the build-up of contaminants in the medium and helps to maintain capacity, flow properties, and general performance. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.Ģ. Capto L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. CIP is a procedure for removal of contaminants such as lipids, endotoxins, nucleic acids, and precipitated or denatured proteins that remain in the packed column after regeneration. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. These columns are easily connected in series to increase bed height to 20 cm.įast results and minimal consumption of sample and buffer consumption through the small bed volume.īioProcess columns packed with the same chromatography resin and using the same linear fluid velocity can produce scalable, reproducible results.ġ. Prepacked with Capto Q strong anion exchange chromatography resin.Ĭonvenient method optimization and parameter screening because of the 10cm bed height. ![]() HiScreen Capto Q is a column packed with a strong anion exchange modern resin, combining speed and capacity. ![]()
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